Colorado Cannabis Testing

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Category: Lab News

PhytaTech is Now Part of Evio Labs

Located in Denver, CO, PhytaTech has been an industry leader in Colorado as both a licensed medical and retail (adult use) testing provider, servicing licensed cultivators and manufacturers of infused products (“MIPs”) since 2014. In September 2017, PhytaTech CO, LLC entered into a technology license agreement with EVIO Labs Colorado to utilize EVIO Labs’ proprietary testing methods.


PhytaTech EVIO Labs is the leading national provider of accredited cannabis testing, providing high quality analytical and consulting services for agricultural and biomedical industries in the United States.


EVIO, Inc. is a life sciences company focused on analyzing cannabis as a means for improving quality of life. The Company provides analytical testing services, advisory services and performs product research in its accredited laboratory testing facilities. The Company’s EVIO Labs division operating coast-to-coast provides state-mandated ancillary services to ensure the safety and quality of the nation’s cannabis supply. EVIO Labs is dedicated to providing clients with superior customer service, reliable results, and world class expertise. 


For more information, visit

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Accreditation to ISO/IES 17025 Standards Through A2LA

PhytaTech is ISO-17025 Certified!


PhytaTech is proud to announce its accreditation to ISO/IES 17025 standards through A2LA (the American Association Laboratories Accreditation). PhytaTech’s scope of work under the ISO-17025 accreditation allows us to perform the following types of tests on cannabis (marijuana) flower, concentrate, topical, and edible products from state licensed medical, recreational, and hemp customers:



Cannabinoid Potency Analysis

Residual Solvent Contamination



E.Coli – qPCR

Salmonella – qPCR

Total Yeast and Molds – Plating     


PhytaTech is one of only eight ISO-17025 accredited laboratories in the United States of America and one of the few in Colorado to include full Potency, RSA and Microbial Contamination testing on it’s scope of work. The ISO standard requires quality management systems, technical systems, and procedures to ensure quality data is delivered to our clients every time. You’ve always noted PhytaTech’s consistent, quick results and now you have the internationally recognized ISO-17025 quality standard behind your data.


We’re proud of our accomplishments and you should be too.


Thank you for your support and Happy Holidays!

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Cannabis Testing Updates

There is a lot changing in Cannabis testing in 2018 and PhytaTech is here to support your testing needs.


Here is a direct link to the MED Adopted rules for both Retail and Medical Marijuana.


Of note, significant changes to the Residual Solvent Contamination test include new solvents and new regulatory limits, mycotoxin testing language is included, pesticide testing of flowers/trim is mentioned, and Residual Solvent Contamination testing and Microbial contamination testing of medical marijuana products will soon be enacted. The MED will send industry bulletins when Mycotoxins, Pesticides and Medical Marijuana Microbial testing will become mandatory.  Medical RSA mandate begins 01.01.2018.


New Residual Solvent Limits Are (Effective 01.01.2018):


Acetone < 1,000 ppm

Butanes (n-butane and iso-butane) < 1,000 ppm

Ethanol < 1,000 ppm (For inhaled products only)

Heptanes < 1,000 ppm

Isopropyl Alcohol < 1,000 ppm

Propane < 1,000 ppm

Benzene < 2 ppm

Toluene < 180 ppm

Pentane < 1,000 ppm

Hexane < 60 ppm

Total Xylenes (m, p, o-xylenes) < 430 ppm


New Pesticide limits are (not effective until MED industry bulletin published):


Abamectin (Avermectins: B1a & B1b) < 70 ppb

Azoxystrobin < 20 ppb

Bifenzate < 20 ppb

Etoxazole < 10 ppb

Imazalil < 40 ppb

Imidacloprid < 20 ppb

Malathion < 50 ppb

Myclobutanil < 40 ppb

Permethrin (isomeric mix) < 40 ppb

Spinosad (Mixture of A & D) < 60 ppb

Spiromesifen < 30 ppb

Spirotetramat < 20 ppb

Tebuconazole < 10 ppb


New mycotoxin limits are (not effective until MED industry bulletin published):

Aflatoxins (B1, B2, G1, and G2) < 20 ppb (Sum of all)

Ochratoxin A < 20 ppb


Remember test batches are to be 4 to 14.5 grams based on the size of your batch and you will need to submit these in one container, already homogenized. There is also rolling testing required after you receive process validation so make sure to submit samples to stay compliant!

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PhytaTech Now Offers a HPLC Method

PhytaTech is proud to announce it now offers a HPLC method, which detects and quantitates Aflatoxins and Ochratoxin A (OTA) in cannabis flowers and concentrates!

The specific molecules included in our internally validated method are:

1.    Aflatoxin B1
2.    Aflatoxin G1
3.    Aflatoxin B2
4.    Aflatoxin G2
5.    Ochratoxin A


Aflatoxins and ochratoxins are secondary metabolites created by certain Aspergillus fungal species and are known carcinogens in extremely low levels. Aflatoxins have been classified as group 1 carcinogens, which is the highest classification a substance can receive. A statistically significant increase in the incidence of hepatocellular (liver) cancer has been linked to aflatoxin exposure at 20 parts per billion (ppb) and higher.


They exhibit their genotoxic effects by altering the human p53 tumor suppressor gene and by binding to albumin (an abundant drug binding protein in blood). Common testing protocols exist in other industries to monitor these dangerous small molecules as food products enter the human consumption chain. The FDA mandates that food products intended for human consumption must not exceed 20 ppb for total aflatoxins. Aflatoxin testing in cannabis is mandated in other states such as Connecticut, Illinois, Massachusetts, Nevada, and New Mexico. These states have limits of 20 ppb for aflatoxins and OTA.


Although OTA testing is not mandated by the FDA, the European Commission has set Ochratoxin A limits from as low as 0.5 ppb to 80 ppb depending on the food classification (cereal-based infant food and licorice extract, for example). OTA has been linked to a specific, fatal kidney disease called Balkan Endemic Nephropathy. Other processes involved with OTA that contribute to its carcinogenicity involve its ability to cause breaks in DNA strands and inhibit DNA repair mechanisms.


These molecules are heat stable and maintain their molecular structures at temperatures below 160°C. Therefore, it is important to know whether these harmful substances exist in any harvest batch prior to any processing of cannabis material. If they are present in cannabis flower, then they can be concentrated during the extraction process to make cannabis concentrates and eventually consumed in cannabis edibles!


Importantly, through genomic testing, we have detected the fungal strains known to produce these molecules in Colorado cannabis flowers! Don’t delay, ensure your products are safe for human consumption.

PhytaTech asks for samples sizes of 1.0 gram for both flowers and concentrates to perform this testing. Feel free to call the lab if you would like to know more about our procedures for Aflatoxin and Ochratoxin testing at PhytaTech. If you’d like to hear more about our value-added test capabilities, tests we’re bringing on line, or our long-term projects, please contact us.

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